Synthetic Biology Research CentreAnaerobe Knock-Out VectorsDescriptionKnock-out vectors are available for the generation of mutants in a range of anaerobes in a classic two-step process by allelic exchange using the counter selection marker codA (cytosine deaminase) or pyrE (orotate phosphoribosyltransferase) [1] codA-based plasmids pMTL-SC7215, pMTL-SC7315 & pMTL-SC7515, previously exemplified in Clostridioides difficile and Clostridium acetobutylicum and based on the pBP1, pCB102 or pIM13 replicons, respectively. [2] pyrE-based plasmids pMTL-ME3 & pMTL-GL3, carrying either the pIM13 replicon or none at all (suicide), respectively. Exemplified in C. acetotobutylicum and C. beijerinckii, respectively. [3] pyrE-based plasmids pMTL-YN3 and pMTL-YN4, based on the pCB102 and pBP1 replicons and exemplified in different strains of Clostridioides difficile. [4] A suicide vector carrying both a pyrE and codA counterselection markers for the direct selection of in-frame deletions in C. acetobutylicum. [5] pyrE-based suicide vector pMTL-JPB22 exemplified in Acetobacterium woodii. [6] A suicide plasmid system for gene knockout in Eubacterium limosum Vectors supplied as a pair at a concentration of 50ng/µl DNA and are ready to be transformed into E. coli. Postage added at check-out.
Vectors are available for academic groups only, companies please contact [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Bacterial Strains Carrying Defined MutantsDescriptionDefined mutants are available in various bacterial species (including, for instance) Clostridium, Cupriavidus, Geobacillus and Methanotrophs. Please identify beforehand exactly which mutants are required by contacting [email protected] or [email protected] with your requirements. Mutants are sent as agar slabs or spore stocks. Postal charges are destination dependent and added at check-out. Queries should be addressed to: [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. CRISPR-Cas9DescriptionMany CRISPR-Cas9 systems suffer from toxicity issues in both E. coli and the intended host as a consequence of overproduction of Cas9. Our Cas9 has a frameshift that results in a truncated Cas9 protein lacking 87 amino acids and is believed to act as a nickase. Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Donor StrainsDescriptionE. coli strains which can be used as conjugative donors. Plasmid DNA propagated in the strains varies in its adenosine and cytosine methylation status due to the presence or absence of functional Dam and Dcm methyltransferases. Each is Mob+ Tra+ and all but S17.1 carry the conjugative plasmid R702 (TcR, SmR, SuR, HgR). Parental strains Express and Stellar are the property of New England Biolabs and CLONTECH, respectively. Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Geobacillus CRISPR-Cas9DescriptionA universal CRISPR_Cas9 gene editing system for broad application in thermophilic Parageobacillus and other anaerobic Gram +ve thermophiles, comprising 3 vectors. The widely used CRISPR/Cas9 nuclease of Streptococcus pyogenes is inactive at temperatures above 42 °C, making its use in thermophiles problematical. To circumvent this issue we have developed three Parageobacillus/E.coli shuttle vector CRISPR/Cas9 systems based on the well-characterized CRISPR/Cas9 systems of Streptococcus thermophiles, stCas91 and stCas93. https://doi.org/10.1021/acssynbio.1c00138 https://doi.org/10.1021/acssynbio.3c00735
The three vectors included in the kit are:- pMTL575555 (stCas91), pMTL675555 (stCas93) and pMTL-RiboCas93. All vectors carry the ColEI and pUB110 origins of replication, a thermostable kanamycin resistance gene and a locus encoding the specific sgRNA while pMTL-RiboCas93 contains a Pgapdh RbxE riboswitch upstream of Cas9. All vectors are supplied at a concentration of 50ng/µl DNA are ready to be transformed into E. coli. The kit costs 300 GBP plus postage at a rate dependent on destination - postage added at check-out. Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. pMTL60000 Modular Plasmid VectorsDescriptionModular shuttle plasmid vectors for use in Parageobacillus and other Gram-positive thermophiles. Comprising a selection of Gram +ve and -ve replicons, antibiotic markers and various different application specific modules. The 20 modules described are distributed as part of a kit comprising seven plasmids (pMTL61221, pMTL62112, pMTL63223, pMTL64224, pMTL65341, pMTL66331, and pMTL67331) from which the other possible combinations of modules can be easily constructed in one or a few cloning step(s) using AscI, FseI, PmeI and SbfI. Plasmids are sent as lyophilised 50ng/µl DNA ready to be transformed into E. coli. and cost 280 GBP per Kit, plus postage at a rate dependent on destination - shipping added at checkout. For further information on the vectors see https://plasmidvectors.com Queries should be addressed to: [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. pMTL70000 Modular Plasmid VectorsDescriptionModular shuttle plasmid vectors for use in Cupriavidus and other Gram-negative aerobes. Comprising a selection of Cupriavidus and E. coli replicons, antibiotic markers and various different application specific modules. KIT 1 The 18 modules originally described are distributed as part of a kit comprising six plasmids (pMTL70621, pMTL71302, pMTL72113, pMTL73414, pMTL74131 & pMTL75511) from which all other possible combinations of modules can be easily constructed in one or a few cloning step(s) using AscI, FseI, PmeI and SbfI. For further information on the vectors see https://plasmidvectors.com Queries should be addressed to: [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. pMTL80000 Modular Plasmid VectorsDescriptionModular shuttle plasmid vectors for use in Clostridium bacteria and other Gram-positive anaerobes. Comprising a selection of Gram +ve and -ve replicons, antibiotic markers and various different application specific modules. KIT 1 The 18 modules originally described are distributed as part of a kit comprising five plasmids (pMTL80110, pMTL82254, pMTL83353, pMTL84422 & pMTL85141) from which the other 395 possible combinations of modules can be easily constructed in one or a few cloning step(s) using AscI, FseI, PmeI and SbfI. KIT 2 Seven plasmids (pMTL89151, pMLT86151, pMTL87151, pMTL81151, pMTL88155, pMTL84131, pMLT82255) comprising five new Gram-positive replicons and the fluorescent marker FAST - for details, see https://plasmidvectors.com Plasmids are sent as lyophilised 50ng/µl DNA ready to be transformed into E. coli. and cost 200 GBP per Kit, plus postage at a rate dependent on destination - shipping added at checkout. For further information on the vectors see https://plasmidvectors.com Queries should be addressed to: [email protected] pMTL80000 modular plasmids at ClosTron http://clostron.com/pMTL80000.php Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. RiboCasDescriptionA universal CRISPR_Cas9 gene editing system for broad application in Clostridia and other anaerobic Gram +ve species, comprising 5 vectors. In RiboCas, Cas9 has been placed under the control of a tightly regulated, theophylline responsive riboswitch. The system overcomes may of the issues relating to Cas9 toxicity in both the shuttle host and intended target. pRECas1 All vectors are supplied at a concentration of 50ng/µl DNA ready to be transformed into E. coli. The kit costs 200 GBP plus postage at a rate dependent on destination – postage added at check-out. External URL: https://plasmidvectors.com Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Specific PlasmidsDescriptionThe plasmid(s) you require should have been agreed beforehand by contacting us at [email protected].
Postal charges are destination dependent and added at check-out. Queries should be addressed to: [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Specific StrainsDescriptionThe SBRC has built up a collection of different bacterial strains, including Clostridium, Cupriavidus, Geobacillus and Methanotrophs. Please contact [email protected] or [email protected] to check the availability of particular strains before placing your order Strains are sent as agar slabs or spore stocks. Postal charges are destination dependent and added at check-out. If you require two strains, then order 2 units at bottom of the page, if 3 strains then order 3 units etc.
Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Transposon VectorsDescriptionA selection of E. coli/Clostridium transposon shuttle vectors carrying a mariner element suitable for random mutant generation in various clostridial species and other Gram-positive bacteria. Those plasmids (pMTL-SC1, pMTL-YZ14, pMTL-CW20) using the PtcdB promoter require the presence of a chromosomal copy of tcdR, either natively present or inserted at the pyrE locus using ACE. pMTL-SC1 – Exemplified in Clostridioides difficile, uses the pBP1 replicon and PtcdB promoter to express the transposase. pMTL-YZ14 – Exemplified in Clostridium acetobutylicum, uses the pCB102 replicon and PtcdB promoter to express the transposase. pMTL-CW20 – Exemplified in Clostridium autoethanogenum, uses the pCB102 replicon and PtcdB promoter to express the transposase. pMTL-CW21– Exemplified in Clostridium sporogenes, uses the pCB102 replicon and the inducible Tet promoter in place of PtcdB , negating with the need for tcdR in the chromosome. pMTL-CW22 - Exemplified in Clostridium sporogenes, uses pCD19 replicon and the inducible Tet promoter in place of the PtcdB promoter, negating the need for tcdR in the chromosome. All vectors are supplied at a concentration of 50ng/µl DNA are ready to be transformed into E. coli. Each vector costs 60 GBP plus postage at a rate dependent on destination - postage added at check-out. Queries should be addressed to: [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. Transposon Vectors CommercialDescriptionA selection of E. coli/Clostridium transposon shuttle vectors carrying a mariner element suitable for random mutant generation in various clostridial species and other Gram-positive bacteria. Those plasmids (pMTL-SC1, pMTL-YZ14, pMTL-CW20) using the PtcdB promoter require the presence of a chromosomal copy of tcdR, either natively present or inserted at the pyrE locus using ACE. pMTL-SC1 – Exemplified in Clostridioides difficile, uses the pBP1 replicon and PtcdB promoter to express the transposase. pMTL-YZ14 – Exemplified in Clostridium acetobutylicum, uses the pCB102 replicon and PtcdB promoter to express the transposase. pMTL-CW20 – Exemplified in Clostridium autoethanogenum, uses the pCB102 replicon and PtcdB promoter to express the transposase. pMTL-CW21– Exemplified in Clostridium sporogenes, uses the pCB102 replicon and the inducible Tet promoter in place of PtcdB , negating with the need for tcdR in the chromosome. pMTL-CW22 - Exemplified in Clostridium sporogenes, uses pCD19 replicon and the inducible Tet promoter in place of the PtcdB promoter, negating the need for tcdR in the chromosome. All vectors are supplied at a concentration of 50ng/µl DNA are ready to be transformed into E. coli. Queries should be addressed to: [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering. |
