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E. coli strains which can be used as conjugative donors.

Plasmid DNA propagated in the strains varies in its adenosine and cytosine methylation status due to the presence or absence of functional  Dam and Dcm methyltransferases.  Each is Mob+ Tra+ and all but S17.1 carry the conjugative plasmid R702 (TcR, SmR, SuR, HgR).  

CA434 = HB101 (R702): F - mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 (SmR) glnV44 λ-)

sExpress = NEB Express (R702): fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 Δ(mcrC-mrr)114::IS10

InterStellar = Stellar (R702): F – ara, Δ(lac-proAB) [Φ80d lacZΔM15], rpsL(str), thi, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, dam, dcm

TopSex = Top10 (R702): mcrA, Δ(mrr-hsdRMS-mcrBC), Phi80lacZ(del)M15, ΔlacX74, deoR, recA1, araD139, Δ(ara-leu)7697, galU, galK, rpsL(SmR), endA1, nupG

S17.1:  pro, res− hsdR17 (rK− mK+) recA− with an integrated RP4-2-Tc::Mu-Km::Tn7, TpR - λpir.

Strains are sent as agar slabs. Postal charges are destination dependent and added at check-out.

Parental strains Express and Stellar are the property of New England Biolabs and CLONTECH, respectively.  

Queries should be addressed to: [email protected] 

https://plasmidvectors.com

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