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A universal CRISPR_Cas9 gene editing system for broad application in Clostridia and other anaerobic Gram +ve species, comprising 5 vectors.

In RiboCas, Cas9 has been placed under the control of a tightly regulated, theophylline responsive riboswitch. The system overcomes may of the issues relating to Cas9 toxicity in both the shuttle host and intended target.

https://doi.org/10.1021/acssynbio.9b00075 

Supplied as a kit, comprising five vectors, incorporating two different riboswitches controlling Cas9 (E & G), and a choice of promoter controlling the expression of the sgRNA, either ParaE (1) or the J23119 promoter (2).  Four of the plasmids are based on the ColE1 replicon, while the fifth uses the low copy number, p15a replication region.

pRECas1
pRECas2
pRECas1-p15a
pRGCas1
pRGCas2

All vectors are supplied at a concentration of 50ng/µl DNA ready to be transformed into E. coli. The kit costs 200 GBP plus postage at a rate dependent on destination – postage added at check-out.

Vectors are available for academic groups only, companies please contact [email protected] 

External URL: https://plasmidvectors.com 

Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering.