Many CRISPR-Cas9 systems suffer from toxicity issues in both E. coli and the intended host as a consequence of overproduction of Cas9.
Our Cas9 has a frameshift that results in a truncated Cas9 protein lacking 87 amino acids and is believed to act as a nickase.
The five vectors supplied are all based on the pCB102 Gram +ve replicon, rely on the Pthl promoter for truncated Cas9 expression and incorporate the listed component parts as (selectable marker - Gram negative replicon - sgRNA promoter):
pMTL431521 - (catP - ColE1/tra - PtcdB)
pMTL431511 - (catP - ColE1/tra - ParaE)
pMTL431211 - (catP - p15a/tra - ParaE)
pMTL432211 - (ermB - p15a/tra - ParaE)
pMTL431541 - (catP - ColE1/tra - PJ23119)
All vectors are supplied at a concentration of 50ng/µl DNA are ready to be transformed into E. coli. The kit costs 5,000 GBP plus postage at a rate dependent on destination - postage added at check-out.
Vectors are available for academic groups only, companies please contact [email protected]
Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering.