A universal CRISPR_Cas9 gene editing system for broad application in thermophilic Parageobacillus and other anaerobic Gram +ve thermophiles, comprising 2 vectors.
The widely used CRISPR/Cas9 nuclease of Streptococcus pyogenes is inactive at temperatures above 42 °C, making its use in thermophiles problematical. To circumvent this issue we have developed two Parageobacillus/E.coli shuttle vector CRISPR/Cas9 systems based on the well-characterized CRISPR/Cas9 systems of Streptococcus thermophiles, stCas91 and stCas93.
Two vectors are included in the kit:-
Both vectors carry the ColEI and pUB110 origins of replication, a thermostable kanamycin resistance gene and a locus encoding the specific sgRNA.
All vectors are supplied at a concentration of 50ng/µl DNA are ready to be transformed into E. coli. The kit costs 200 GBP plus postage at a rate dependent on destination - postage added at check-out.
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