Anaerobe Knock-Out Vectors DescriptionKnock-out vectors are available for the generation of mutants in a range of anaerobes in a classic two-step process by allelic exchange using the counter selection marker codA (cytosine deaminase) or pyrE (orotate phosphoribosyltransferase) [1] codA-based plasmids pMTL-SC7215, pMTL-SC7315 & pMTL-SC7515, previously exemplified in Clostridioides difficile and Clostridium acetobutylicum and based on the pBP1, pCB102 or pIM13 replicons, respectively. [2] pyrE-based plasmids pMTL-ME3 & pMTL-GL3, carrying either the pIM13 replicon or none at all (suicide), respectively. Exemplified in C. acetotobutylicum and C. beijerinckii, respectively. [3] pyrE-based plasmids pMTL-YN3 and pMTL-YN4, based on the pCB102 and pBP1 replicons and exemplified in different strains of Clostridioides difficile. [4] A suicide vector carrying both a pyrE and codA counterselection markers for the direct selection of in-frame deletions in C. acetobutylicum. [5] pyrE-based suicide vector pMTL-JPB22 exemplified in Acetobacterium woodii. [6] A suicide plasmid system for gene knockout in Eubacterium limosum Vectors supplied as a pair at a concentration of 50ng/µl DNA and are ready to be transformed into E. coli. Postage added at check-out.
Vectors are available for academic groups only, companies please contact [email protected] Please note: It is the responsibility of the buyer to make sure that prior to purchase that the required goods are accepted into the destination country and that any required documentation (including import permits) are in place. Refunds cannot be processed for any orders returned to us due to customs issues. Please contact [email protected] for any questions relating to ordering.
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