E. coli strains which can be used as conjugative donors.
Plasmid DNA propagated in the strains varies in its adenosine and cytosine methylation status due to the presence or absence of functional Dam and Dcm methyltransferases. Each is Mob+ Tra+ and all but S17.1 carry the conjugative plasmid R702 (TcR, SmR, SuR, HgR).
sExpress = NEB Express (R702): fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 ?(mcrC-mrr)114::IS10
Strains are sent as agar slabs. Postal charges are destination dependent and added at check-out.
Parental strains Express and Stellar are the property of New England Biolabs and CLONTECH, respectively.
Queries should be addressed to: [email protected]
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